abcb5 antibody  (Santa Cruz Biotechnology)

Journal: Regenerative Therapy

Article Title: Conditioned media of stem cells from human exfoliated deciduous teeth contain factors related to extracellular matrix organization and promotes corneal epithelial wound healing

doi: 10.1016/j.reth.2025.03.002

Figure Lengend Snippet: Immunohistochemical findings of limbal stem cell markers in the limbal epithelium of chronic graft-versus-host disease (cGVHD) mice. Sections of the limbus from syngeneic control mice and cGVHD mice treated with vehicle, stem cells from human exfoliated deciduous teeth-conditioned medium (SHED-CM), immortalized-SHED-CM (IM-SHED-CM), or >3.5 kD fractionated components were stained for ATP-binding cassette sub-family B member 5 (ABCB5) (green) (a) or p63 (green) (b) (n = 5–6, per group). The corneal epithelium is located above the white dotted line. Data are presented as the mean ± standard error of the mean. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. One-way analysis of variance with Tukey–Kramer's post-hoc test was used for the analysis. Scale bar, 10 μm.

Article Snippet: This process was applied to antibodies against p63 (dilution of 1:500, sc-25268, monoclonal, Santa Cruz Biotechnology, Dallas, TX), ATP-binding cassette sub-family B member 5 (ABCB5) (dilution of 1:500, bs-1604R, Bioss Antibodies, Woburn, MA), 4-hydrogy-2-nonenal (4-HNE) (dilution of 1:100, HNEJ-2, Japan Institute for the Control Aging, Shizuoka, Japan), or 8-hydroxy-2′-deoxyguanosine (8-OHdG) (dilution of 1:50, MOG-020P, N45.1, Genox, Torrance, CA).

Techniques: Immunohistochemical staining, Control, Staining, Binding Assay

Journal: Current Issues in Molecular Biology

Article Title: Valproic Acid as a Histone Deacetylase Inhibitor Induces ABCB1 Overexpression and De Novo ABCB5 Expression in HeLa Cells

doi: 10.3390/cimb47090749

Figure Lengend Snippet: Protein sequence detected by Novus ABCB5 monoclonal antibody and its comparison to variants 1 and 2. The immunogen comprises 194 amino acids ( A ). ABCB5 protein immunodetection in HeLa cells treated with 5 mM VPA ( B ). NT: non-treated.

Article Snippet: The cells were fixed with 100% ice-cold methanol, blocked with 5% normal goat serum, and incubated overnight at 4 °C with a 1:200 dilution of primary ABCB5 monoclonal antibody (Novus Biologicals, Littleton, CO, USA).

Techniques: Sequencing, Comparison, Immunodetection

Journal: Oncology Research

Article Title: Identifying ATP-Binding Cassette Member B5 as a New Biomarker for Oral Squamous Cell Carcinoma

doi: 10.32604/or.2025.064276

Figure Lengend Snippet: ABCB5 is highly expressed in oral cancer cell lines: ( a–e ) The mRNA and protein expressions of ABCB5 were measured in HOK, CAL27, and HSC-3 using qRT-PCR, western blot, and ICC. ( a ) ABCB5 mRNA level in HOK, CAL27, and HSC-3 was detected by qRT-PCR, n = 3 qRT-PCR runs. ( b,c ) The ABCB5 protein level in HOK, CAL27, and HSC-3 was measured by western blot ( b ), the density of ABCB5 was quantified by ImageJ, and β-actin was used as a loading control, n = 3 ( c ). ( d ) Representative images of HOK, CAL27, and HSC-3 cells stained with ABCB5 (red), scale bar: 100 μm. ( e ) The ABCB5-positive areas, n = 5 fields. Data represent the mean ± SD; one-way ANOVA was used ( a,c,e )

Article Snippet: After blocking with 5% skimmed milk, the primary ABCB5 antibody (Santa Cruz Biotechnology, Inc., sc-515910, Shanghai, China) was incubated with a dilution of 1:500 for an entire night at 4°C.

Techniques: Quantitative RT-PCR, Western Blot, Control, Staining

Journal: Oncology Research

Article Title: Identifying ATP-Binding Cassette Member B5 as a New Biomarker for Oral Squamous Cell Carcinoma

doi: 10.32604/or.2025.064276

Figure Lengend Snippet: ABCB5 knockdown inhibits the migration and invasion of 2D OSCC cells: ( a–f ) ABCB5 was knocked down in the CAL27 cells using siABCB5, and the migration and invasion ability of the CAL27 cells were evaluated. ( a ) ABCB5 mRNA levels in CAL27 cells transfected with siABCB5 were evaluated using qRT-PCR, n = 3 qRT-PCR runs. ( b ) Representative images of CAL27 cells stained with ABCB5 (red), scale bar: 100 μm. ( c ) The ABCB5-positive areas, n = 5 fields. ( d–f ) The number of migrated ( d(upper),e ) and invaded ( d(lower),f ) CAL27 cells were imaged, scale bars: 100 μm ( d ) and counted, n = 5 fields ( e,f ). Data represent the mean ± SD; one-way ANOVA was used ( a,c,e,f )

Article Snippet: After blocking with 5% skimmed milk, the primary ABCB5 antibody (Santa Cruz Biotechnology, Inc., sc-515910, Shanghai, China) was incubated with a dilution of 1:500 for an entire night at 4°C.

Techniques: Knockdown, Migration, Transfection, Quantitative RT-PCR, Staining

Journal: Oncology Research

Article Title: Identifying ATP-Binding Cassette Member B5 as a New Biomarker for Oral Squamous Cell Carcinoma

doi: 10.32604/or.2025.064276

Figure Lengend Snippet: ABCB5 knockdown inhibits the migration and invasion of 3D OSCC spheroid: ( a–d ) The migration and invasion ability of 3D OSCC spheroid were evaluated in the CAL27 cells after transfected with siABCB5. ( a,b ) Representative images of migrated ( a ) and invaded ( b ) 3D OSCC spheroid, scale bars: 100 μm ( a,b ), and the area of cells migrated or invaded were calculated, n = 5 fields ( c,d ). Data represent the mean ± SD; one-way ANOVA was used ( c,d )

Article Snippet: After blocking with 5% skimmed milk, the primary ABCB5 antibody (Santa Cruz Biotechnology, Inc., sc-515910, Shanghai, China) was incubated with a dilution of 1:500 for an entire night at 4°C.

Techniques: Knockdown, Migration, Transfection

Journal: Oncology Research

Article Title: Identifying ATP-Binding Cassette Member B5 as a New Biomarker for Oral Squamous Cell Carcinoma

doi: 10.32604/or.2025.064276

Figure Lengend Snippet: ABCB5 Knockdown suppresses TGF-β-induced EMT: ( a ) The correlations between ABCB5 and epithelial marker E-cadherin and mesenchymal markers (N-cadherin and Vimentin) were observed by qRT-PCR in CAL27 (left) and HSC-3 (right) cells, which were treated with TGF-β in different concentrations, n = 3 qRT-PCR runs. ( b,c ) The mRNA expressions of epithelial marker E-cadherin ( b ) and mesenchymal markers (N-cadherin and Vimentin) ( c ) in CAL27 cells were analyzed using qRT-PCR, n = 3 qRT-PCR runs. ( d,e ) Representative images of CAL27 cells with or without TGF-β treatment were stained with E-cadherin, scale bar: 100 μm ( d ), and the positive areas of E-cadherin were calculated, n = 5 fields ( e ). ( f,g ) Representative images of CAL27 cells with or without TGF-β treatment were stained with Vimentin, scale bar: 100 μm ( f ), and the Vimentin-positive areas were quantified, n = 5 fields ( g ). Data represent the mean ± SD; one-way ANOVA was used ( b,c,e,g ); two-way ANOVA was used ( a ).

Article Snippet: After blocking with 5% skimmed milk, the primary ABCB5 antibody (Santa Cruz Biotechnology, Inc., sc-515910, Shanghai, China) was incubated with a dilution of 1:500 for an entire night at 4°C.

Techniques: Knockdown, Marker, Quantitative RT-PCR, Staining

Journal: Oncology Research

Article Title: Identifying ATP-Binding Cassette Member B5 as a New Biomarker for Oral Squamous Cell Carcinoma

doi: 10.32604/or.2025.064276

Figure Lengend Snippet: ABCB5 was highly expressed in the 4NQO-induced OSCC model in vivo: ( a–g ) The 4NQO carcinogen mouse model was established, and the expressions of ABCB5 and EMT markers were detected. ( a ) Schematic explains the experimental design of the 4NQO carcinogen mouse model. ( b ) Representative images of H&E staining, scale bar: 100 μm. ( c ) Representative image of tongue tissues stained with ABCB5, scale bar: 100 μm. ( d ) Quantification of ABCB5-positive areas, n = 5 fields. ( e ) mRNA expression of ABCB5 detected by qRT-PCR, n = 3 qRT-PCR runs. ( f,g ) mRNA levels of epithelial marker E-cadherin ( f ) and mesenchymal markers (N-cadherin and Vimentin) ( g ) were evaluated by qRT-PCR, n = 3 qRT-PCR runs, n = 5–8 mice per group. Data represent the mean ± SD; one-way ANOVA was used ( d,e,f,g )

Article Snippet: After blocking with 5% skimmed milk, the primary ABCB5 antibody (Santa Cruz Biotechnology, Inc., sc-515910, Shanghai, China) was incubated with a dilution of 1:500 for an entire night at 4°C.

Techniques: In Vivo, Staining, Expressing, Quantitative RT-PCR, Marker